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Blebbistatin (CAS 856925-71-8): The Gold Standard Myosin II Inhibitor for Precision Research

In advanced life science research, the precision of your molecular tools dictates the reliability of your data. (-)-Blebbistatin (CAS No. 856925-71-8) is an essential, high-selectivity bioactive small molecule for scientists exploring the frontiers of cell biology, neuroscience, and mechanobiology.

Finetech Industry Limited specializes in supplying high-purity, research-grade (-)-Blebbistatin, ensuring your experiments are backed by consistent and reproducible results.

🔬 Chemical Profile & Specifications

The biological activity of Blebbistatin is highly dependent on its stereochemistry. As the active enantiomer, (-)-Blebbistatin offers superior potency compared to the racemic mixture.1

Property Specification

Chemical Name (-)-Blebbistatin

CAS Number 856925-71-8

Molecular Formula $C_{18}H_{16}N_{2}O_{2}$

Molecular Weight 292.33

Purity ≥98% (HPLC verified)

Solubility DMSO

⚙️ Mechanism of Action: How it Works

Unlike general inhibitors, (-)-Blebbistatin offers a sophisticated mode of action. It selectively targets non-muscle myosin II by stabilizing the myosin–ADP–Pi complex.

Force Inhibition: It prevents the power stroke, effectively "locking" the motor without detaching it from the actin.

Structural Integrity: It inhibits contractility without disrupting the underlying actin filament network.

Reversibility: Its effects can be washed out, allowing for dynamic, "start-stop" temporal studies of cellular tension.

🧬 Key Research Applications

1. Cell Biology & Cytoskeletal Dynamics

Investigate the fundamental forces of life. (-)-Blebbistatin is the preferred tool for studying:

Cytokinesis: Visualizing cell division and contractile ring formation.

Cell Migration: Understanding how cells crawl and adhere to 2D/3D matrices.

Morphology: Probing the relationship between actomyosin tension and cell shape.

2. Neuroscience & Developmental Biology

Mechanical tension is a primary driver of neural growth. Use this inhibitor to study:

Axon Guidance: How growth cones navigate through mechanical cues.

Synaptic Plasticity: The role of myosin in dendritic spine remodeling.

Morphogenesis: Tissue-level folding and development driven by cellular forces.

3. Mechanobiology & Tissue Engineering

In the era of organoids and "lab-on-a-chip" technology, controlling force is vital:

Traction Force Microscopy (TFM): Measuring the exact forces cells exert on substrates.

Stem Cell Differentiation: Exploring how substrate stiffness and internal tension dictate cell fate.

✅ Why Source from Finetech Industry Limited?

When you choose Finetech, you are choosing a partner dedicated to scientific excellence. Our (-)-Blebbistatin is characterized by:

Enantiomeric Purity: Guaranteed (-)-form for maximum biological efficacy.

Full Analytical Support: Every batch is shipped with NMR, MS, and HPLC reports.

Proven Stability: Optimized packaging to prevent light-sensitive degradation.

Global Logistics: Efficient, temperature-controlled shipping to research institutes and pharma labs worldwide

📖 Recommended References

Straight, A. F. et al. Dissecting temporal and spatial control of cytokinesis. Science, 2003.

Kovács, M. et al. Mechanism of blebbistatin inhibition of myosin II. JBC, 2004.

Even-Ram, S. et al. Myosin II regulates cell migration and adhesion dynamics. Nature Cell Biology, 2007.

🚀 Order Now for Your Next Project

Ready to elevate your mechanobiology research? Contact Finetech Industry Limited today for a quote or technical consultation.

Keywords: Myosin II Inhibitor, (-)-Blebbistatin, CAS 856925-71-8, Actomyosin contractility, Cell biology reagents, Mechanobiology tools, Finetech Industry Limited.