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Xylene Cyanol FF Supplier – Finetech Industry Limited | CAS 4463-44-9, ≥80% BR Grade and Bulk Supply

1. Product Overview

Xylene Cyanol FF (CAS 4463-44-9) is a synthetic blue-purple dye widely used as a tracking dye in molecular biology and electrophoresis applications. It is particularly important in nucleic acid electrophoresis, where it helps researchers monitor the progress of DNA and RNA sample migration through agarose or polyacrylamide gels.

As an electrophoresis tracking dye, Xylene Cyanol FF provides a visible colored marker that moves through the gel during electrophoresis. This allows researchers to follow sample migration and estimate the progress of electrophoretic separation.

Xylene Cyanol FF is also known as Xylene Cyanol, Xylene Blue FF, and C.I. 43535. Its distinctive color and suitability for laboratory electrophoresis make it a commonly used reagent in molecular biology research.

Finetech Industry Limited supplies Xylene Cyanol FF with a specified dye content of ≥80.0% (BR Grade), suitable for laboratory research and electrophoresis-related applications.

2. Product Specifications

ParameterSpecification
Product NameXylene Cyanol FF
SynonymsXylene Cyanol; Xylene Blue FF; C.I. 43535
CAS Number4463-44-9
Molecular FormulaC₂₅H₂₉N₂NaO₇S₂
Molecular Weight556.62 g/mol
AppearancePurple powder
Purity / Dye Content≥80.0%
GradeBR Grade
IdentificationIR Spectrum
Available Packaging5 g, 10 g, 100 g, 1 kg
Bulk Supply5–25 kg and larger quantities available upon inquiry

3. Chemical Characteristics and Functional Properties

Xylene Cyanol FF is an anionic sulfonated dye characterized by an extended conjugated molecular structure, which contributes to its distinctive visible color.

Its sulfonate groups contribute to its water compatibility, while its chromophoric structure provides strong visible coloration under laboratory conditions.

In electrophoresis applications, Xylene Cyanol FF functions primarily as a visual tracking indicator rather than as a nucleic acid stain. It moves through the electrophoretic gel along with the sample and allows researchers to monitor the progress of the separation process.

Its migration behavior depends on experimental conditions, including gel composition, gel concentration, electrophoresis buffer, and operating conditions.

4. Research and Laboratory Applications

4.1 DNA Gel Electrophoresis

One of the principal applications of Xylene Cyanol FF is as a tracking dye in DNA agarose gel electrophoresis.

During electrophoresis, the dye migrates through the gel and provides a visible reference for monitoring the movement of DNA samples. Researchers can use the colored dye front to help determine when to stop electrophoresis and reduce the risk of samples migrating beyond the desired separation region.

Xylene Cyanol FF is therefore a useful component in DNA sample loading and electrophoresis workflows.

4.2 RNA Electrophoresis

Xylene Cyanol FF is also used in laboratory workflows involving RNA electrophoresis.

As a tracking dye, it helps researchers visually monitor electrophoretic migration during RNA sample separation. It can be incorporated into suitable RNA loading dye formulations to provide a visible indicator during gel electrophoresis.

Its use supports routine molecular biology procedures involving RNA analysis and sample preparation.

4.3 Polyacrylamide Gel Electrophoresis (PAGE)

Xylene Cyanol FF is relevant to polyacrylamide gel electrophoresis, where tracking dyes are used to monitor sample migration during electrophoretic separation.

Depending on gel composition and experimental conditions, the dye can provide a useful visual reference for tracking electrophoresis progress.

This application makes Xylene Cyanol FF relevant to protein and nucleic acid laboratory workflows that use suitable electrophoresis systems. It should be noted that the dye itself is a migration indicator, not a protein-specific detection reagent.

4.4 Electrophoresis Loading Buffer Formulation

Xylene Cyanol FF is commonly incorporated into electrophoresis loading buffer formulations.

Loading buffers containing tracking dyes allow researchers to visualize sample application and monitor electrophoretic migration. Xylene Cyanol FF can be used alone or in combination with other tracking dyes, depending on the formulation and analytical requirements.

Its use is particularly relevant to the preparation of laboratory reagents for DNA and RNA electrophoresis.

4.5 Molecular Biology and Biochemical Research

Beyond routine electrophoresis, Xylene Cyanol FF is useful in general molecular biology laboratory workflows where visible tracking indicators are required.

It supports experimental procedures involving nucleic acid sample preparation, gel-based separation, and electrophoresis method development.

Its recognizable color and established laboratory use make it a practical reagent for research laboratories, biotechnology organizations, and educational institutions.

5. Xylene Cyanol FF Supply from Finetech Industry Limited

Finetech Industry Limited supplies Xylene Cyanol FF (CAS 4463-44-9) with a specified dye content of ≥80.0% (BR Grade).

Our product specifications include:

  1. Dye Content: ≥80.0%
  2. Grade: BR Grade
  3. Appearance: Purple powder
  4. Identification: IR Spectrum
  5. Small Packaging: 5 g, 10 g, and 100 g
  6. Laboratory Packaging: 1 kg
  7. Bulk Supply: 5–25 kg and larger quantities available upon inquiry

Finetech supports different procurement requirements, from small laboratory quantities to larger-volume orders for qualified research organizations, biotechnology companies, laboratory reagent distributors, and industrial customers.

For bulk requirements, customized packaging, product availability, or quotations, please contact our team.

6. Why Choose Finetech Industry Limited?

Finetech Industry Limited supplies research chemicals, biochemical reagents, molecular biology materials, and specialty chemical products to international customers.

Our service advantages include:

  1. Research Reagent Portfolio: A broad selection of chemicals for molecular biology, biochemical, and laboratory research.
  2. Specified Product Quality: Products supplied according to defined quality and identification requirements.
  3. Analytical Support: Appropriate analytical identification, including IR testing where applicable.
  4. Flexible Packaging: Options for small research quantities and larger-volume procurement.
  5. Bulk Supply Capability: Support for 5–25 kg orders and larger supply inquiries.
  6. Documentation Support: COA and SDS can be provided as applicable.
  7. Professional Customer Service: Responsive assistance with technical inquiries, quotations, and order coordination.

We aim to provide dependable research chemical supply solutions for laboratories, biotechnology companies, and international chemical distributors.

7. Contact Finetech Industry Limited

For product specifications, availability, quotations, and bulk supply inquiries, please contact Finetech Industry Limited.

Product: Xylene Cyanol FF

CAS Number: 4463-44-9

Dye Content: ≥80.0%

Grade: BR Grade

Appearance: Purple powder

Identification: IR Spectrum

Packaging: 5 g, 10 g, 100 g, 1 kg

Bulk Supply: 5–25 kg and larger quantities available upon inquiry

Website: https://www.finetechnology-ind.com/

Finetech Industry Limited – Your Partner for Research Chemicals and Specialty Chemical Supply.